In vitro SCREENING FOR RESISTANCE TO PROLINE ANALOGUES IN ORCHARDGRASS Dactylis glomerata L
نویسنده
چکیده
Plant regeneration capacity of orchardgrass leaf explants cultured in vitro in the presence of 0.01, 0.1, 1.0 and 10.0mM hydroxyproline (Hyp) or azetidin-2-carboxylic acid (A2CA) was determined. Hyp inhibited to a lower extent than A2CA formation of somatic embryos, respectively plants. Hyp also induced relatively insignificant changes of free amino acid content in the leaves of the regenerants. Overproduction (3–10 times) of lysine, serine, arginine was detected in 3 plants obtained after treatment with 0.01 mM A2CA. The intracellular free proline content estimates suggest different mechanisms of resistance to proline analogues.
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